August Krogh on Tour: Systems analysis of Insulin and Exercise Actions in Muscle and Fat

We are pleased to announce that Professor David James will join us at the Danish Diabetes Academy's Annual Day as a part of "August Krogh on Tour".

Please sign up for this event, if you will only participate in the talk with David James Tuesday 12 November 16:00 - 17:30. If you would like to participate in the entire Annual Day, please sign up for the Annual Day instead.

Abstract
Defining cellular responses to acute perturbations is of central interest in biology. We have exploited altered protein phosphorylation as a key response output as it is rapid, dynamic and quantifiable on a global scale. In light of our main interest in metabolism we have focused on the phosphoproteome of core metabolic tissues such as muscle and fat and how they respond to the major metabolic perturbations such as exercise and insulin. We have identified a vast array of phosphorylation changes in both cases. Surprisingly the majority of these phosphorylation events can neither be functionally accounted for nor ascribed to an upstream kinase. Thus, we refer to these as the ‘dark phosphoproteome’. By focusing on known core regulators of exercise and insulin, such as AMPK and Akt, we have defined novel features and substrates of these regulatory nodes. This has provided new insights into insulin and exercise action and offers a wealth of opportunities for future investigations.